Services Histology

Microanatomy of cells, tissues, and organs

Equipped with cutting edge technologies, and staffed by a CSMLS certified histotechnologist, the Histology Facility provides both routine and specialized histological support to the Lower Mainland research community, including specific services listed below, and assay development and optimization.

Processing and Embedding

Parrafin embedding, OCT embedding for cryosectioning, methylmethacrylate for electron microscopy.

Sectioning

Paraffin, frozen, methylmethacrylate, decalcification (acid)

Staining

Histological staining (see below), immunohistochemistry (peroxidase and/or alkaline phosphatase), immunofluorescence, TUNEL staining, in situ hybridization (ISH).

Histological Stains Available

General morphology: Hematoxylin and Eosin, Toluidine Blue, Giemsa, TTC staining.

Carbohydrates: Periodic Acid-Schiff (PAS), PAS/diastase (glycogen), Alcian blue, Alcian blue with PAS.

Connective Tissue: Trichromes (Masson, Gomori), Movat’s Pentachrome, Gomori’s Aldehyde Fuchsin, Verhoeff/Van Gieson, Picrosirius Red, Gomori’s silver impregnation

Lipids: Oil Red O

Microorganisms: Gram, Brown and Brenn, Zeihl-Neelsen, Grocott

Nucleic Acids: Feulgen

Intracellular Granules: Masson Fontana (melanin), Von Kossa (calcium), amyloid, Perl’s Prussian Blue (hemosiderin).

Hematology: Hansel’s (Eosinophils), Wright’s