White blood cell differentials enrich whole blood expression data in the context of acute cardiac allograft rejection.

TitleWhite blood cell differentials enrich whole blood expression data in the context of acute cardiac allograft rejection.
Publication TypeJournal Article
Year of Publication2012
AuthorsShannon, CP, Hollander, Z, Wilson-McManus, J, Balshaw, R, Ng, RT, McMaster, R, McManus, BM, Keown, PA, Tebbutt, SJ
JournalBioinform Biol Insights
Volume6
Pagination49-61
Date Published2012
ISSN1177-9322
Abstract

Acute cardiac allograft rejection is a serious complication of heart transplantation. Investigating molecular processes in whole blood via microarrays is a promising avenue of research in transplantation, particularly due to the non-invasive nature of blood sampling. However, whole blood is a complex tissue and the consequent heterogeneity in composition amongst samples is ignored in traditional microarray analysis. This complicates the biological interpretation of microarray data. Here we have applied a statistical deconvolution approach, cell-specific significance analysis of microarrays (csSAM), to whole blood samples from subjects either undergoing acute heart allograft rejection (AR) or not (NR). We identified eight differentially expressed probe-sets significantly correlated to monocytes (mapping to 6 genes, all down-regulated in ARs versus NRs) at a false discovery rate (FDR) ≤ 15%. None of the genes identified are present in a biomarker panel of acute heart rejection previously published by our group and discovered in the same data***.

DOI10.4137/BBI.S9197
Alternate JournalBioinform Biol Insights
PubMed ID22550401
PubMed Central IDPMC3329187