|Title||Vasopressin decreases sepsis-induced pulmonary inflammation through the V2R.|
|Publication Type||Journal Article|
|Year of Publication||2008|
|Authors||Boyd, JH, Holmes, CL, Wang, Y, Roberts, H, Walley, KR|
|Date Published||2008 Nov|
|Keywords||Animals, Cell Culture Techniques, Disease Models, Animal, Epithelial Cells, Humans, Interleukin-6, Lipopolysaccharides, Mice, NF-kappa B, Pneumonia, Pulmonary Alveoli, Receptors, Vasopressin, Sepsis, Signal Transduction, Vasoconstrictor Agents, Vasopressins|
UNLABELLED: The early use of vasopressors in sepsis has been associated with a decrease in immune activation independent of hemodynamic effects, although the mechanism behind this remains unclear. We hypothesize that low dose vasopressin will reduce the pulmonary inflammation associated with sepsis. Our aims were to (1) determine whether vasopressin reduces lipopolysaccharide (LPS)-induced pulmonary inflammation and (2) determine which vasopressin receptor is responsible for pulmonary immune modulation. Mice were treated with intraperitoneal LPS to induce both systemic and pulmonary inflammation. Vasopressin or saline was infused via peritoneal pump and interleukin 6 (IL-6) in lung and serum was measured at 6h. NF-kappaB activation as was determined in the lung through immunoblotting total and phospho-IkappaB. Hemodynamic data was also obtained at the 6h mark. In a separate series of experiments mice received both LPS and vasopressin infusion following pretreatment with vasopressin receptor antagonists to V1R, V2R and OTR. Low dose LPS dramatically raises both serum IL-6 and pulmonary levels of IL-6 and phospho-IkappaB despite no significant changes in mean arterial pressure at 6h. Compared to saline, vasopressin infusion significantly decreases both the pulmonary IL-6 levels and phospho-IkappaB in LPS treated mice without raising arterial pressure. Pretreatment with V2R antagonist results in complete attenuation of vasopressin's immunosuppressive effects, with restoration of pulmonary IL-6 and phospho-IkappaB levels to those seen with LPS alone.CONCLUSIONS: Vasopressin exerts a local anti-inflammatory effect on the lung through the V2R in a model of sepsis.