Genetic modifiers of lung disease in cystic fibrosis.

TitleGenetic modifiers of lung disease in cystic fibrosis.
Publication TypeJournal Article
Year of Publication2005
AuthorsDrumm, ML, Konstan, MW, Schluchter, MD, Handler, A, Pace, R, Zou, F, Zariwala, M, Fargo, D, Xu, A, Dunn, JM, Darrah, RJ, Dorfman, R, Sandford, AJ, Corey, M, Zielenski, J, Durie, P, Goddard, K, Yankaskas, JR, Wright, FA, Knowles, MR
Corporate AuthorsGene Modifier Study Group
JournalN Engl J Med
Volume353
Issue14
Pagination1443-53
Date Published2005 Oct 6
ISSN1533-4406
KeywordsAdolescent, Adult, Child, Cystic Fibrosis, Cystic Fibrosis Transmembrane Conductance Regulator, DNA Replication, Female, Forced Expiratory Volume, Genotype, Humans, Linkage Disequilibrium, Logistic Models, Lung Diseases, Male, Middle Aged, Phenotype, Polymorphism, Genetic, Severity of Illness Index, Transforming Growth Factor beta
Abstract

BACKGROUND: Polymorphisms in genes other than the cystic fibrosis transmembrane conductance regulator (CFTR) gene may modify the severity of pulmonary disease in patients with cystic fibrosis.METHODS: We performed two studies with different patient samples. We first tested 808 patients who were homozygous for the DeltaF508 mutation and were classified as having either severe or mild lung disease, as defined by the lowest or highest quartile of forced expiratory volume in one second (FEV1), respectively, for age. We genotyped 16 polymorphisms in 10 genes reported by others as modifiers of disease severity in cystic fibrosis and tested for an association in patients with severe disease (263 patients) or mild disease (545). In the replication (second) study, we tested 498 patients, with various CFTR genotypes and a range of FEV1 values, for an association of the TGFbeta1 codon 10 CC genotype with low FEV1.RESULTS: In the initial study, significant allelic and genotypic associations with phenotype were seen only for TGFbeta1 (the gene encoding transforming growth factor beta1), particularly the -509 and codon 10 polymorphisms (with P values obtained with the use of Fisher's exact test and logistic regression ranging from 0.006 to 0.0002). The odds ratio was about 2.2 for the highest-risk TGFbeta1 genotype (codon 10 CC) in association with the phenotype for severe lung disease. The replication study confirmed the association of the TGFbeta1 codon 10 CC genotype with more severe lung disease in comparisons with the use of dichotomized FEV1 for severity status (P=0.0002) and FEV1 values directly (P=0.02).CONCLUSIONS: Genetic variation in the 5' end of TGFbeta1 or a nearby upstream region modifies disease severity in cystic fibrosis.

DOI10.1056/NEJMoa051469
Alternate JournalN. Engl. J. Med.
PubMed ID16207846
Grant ListHL68890 / HL / NHLBI NIH HHS / United States
RR00046 / RR / NCRR NIH HHS / United States
RR00059 / RR / NCRR NIH HHS / United States