Enhanced Enteroviral Infectivity via Viral Protease-Mediated Cleavage of GAB1

TitleEnhanced Enteroviral Infectivity via Viral Protease-Mediated Cleavage of GAB1
Publication TypeJournal Article
Year of Publication2015
AuthorsDeng, H, Fung, G, Shi, J, Xu, S, Wang, C, Yin, M, Hou, J, Zhang, J, Jin, ZG, Luo, H
JournalFASEB J
Volume29
Issue11
Pagination4523-31
Date Published11/2015
ISSNPrint: 0892-6638; Online: 1530-6860
Abstract

Coxsackievirus B3 (CVB3), an important human causative pathogen for viral myocarditis, pancreatitis, and meningitis, has evolved different strategies to manipulate the host signaling machinery to ensure successful viral infection. We previously revealed a crucial role for the ERK1/2 signaling pathway in regulating viral infectivity. However, the detail mechanism remains largely unknown. Grb2-associated binder 1 (GAB1) is an important docking protein responsible for intracellular signaling assembly and transduction. In this study, we demonstrated that GAB1 was proteolytically cleaved after CVB3 infection at G175 and G436 by virus-encoded protease 2A(pro), independent of caspase activation. Knockdown of GAB1 resulted in a significant reduction of viral protein expression and virus titers. Moreover, we showed that virus-induced cleavage of GAB1 is beneficial to viral growth as the N-terminal proteolytic product of GAB1 (GAB1-N1-174) further enhances ERK1/2 activation and promotes viral replication. Our results collectively suggest that CVB3 targets host GAB1 to generate a GAB1-N1-174 fragment that enhances viral infectivity, at least in part, via activation of the ERK pathway. The findings in this study suggest a novel mechanism that CVB3 employs to subvert the host signaling and facilitate consequent viral replication.

DOI10.1096/fj.15-274829