The effect of cigarette smoking on the bone marrow.

TitleThe effect of cigarette smoking on the bone marrow.
Publication TypeJournal Article
Year of Publication1997
AuthorsTerashima, T, Wiggs, B, English, D, Hogg, JC, van Eeden, SF
JournalAm J Respir Crit Care Med
Volume155
Issue3
Pagination1021-6
Date Published1997 Mar
ISSN1073-449X
KeywordsAnimals, Bone Marrow, Bone Marrow Cells, Bromodeoxyuridine, Cell Division, Female, Immunoenzyme Techniques, Leukocyte Count, Neutrophil Activation, Neutrophils, Rabbits, Smoking, Time Factors
Abstract

Chronic cigarette smoking produces a 20 to 25% increase in the number of peripheral blood leukocytes, and there is increasing evidence that these leukocytes are activated in the lung by the inhalation of cigarette smoke. The present study was designed to measure the effect of cigarette smoke inhalation on the rate of production and release of polymorphonuclear leukocytes (PMN) from the bone marrow into the peripheral blood. The thymidine analogue 5'-bromo-2'-deoxyuridine (BrdU) was used to pulse-label the dividing cells in the marrow of rabbits, measure their appearance in the peripheral blood, and calculate the time that PMN spend in the mitotic and postmitotic pools of the bone marrow. Comparison of animals exposed to 2 wk of cigarette smoke (n = 8) with sham-exposed controls (n = 9) showed that smoking decreased the mean transit time of PMNBrdU through the bone marrow from 97.3 +/- 3.0 h to 89.6 +/- 5.8 h (p < 0.001) by reducing the transit time of PMN in the postmitotic pool from 66.7 +/- 3.9 h to 53.7 +/- 0.7 h (p < 0.001). Both the mitotic (p < 0.05) and postmitotic (p < 0.05) pools of PMN increased in size following cigarette-smoke exposure. We conclude that chronic cigarette smoking stimulates the bone marrow, increases the size of the mitotic and postmitotic pools of PMN, and reduces the time PMN spend in the postmitotic pool in the marrow. These changes may contribute to the leukocytosis seen in cigarette smokers.

DOI10.1164/ajrccm.155.3.9116981
Alternate JournalAm. J. Respir. Crit. Care Med.
PubMed ID9116981