Histology Core

The Histology Facility at the Centre for Heart Lung Innovation plays an integral role in the Centre’s research programs. Equipped with cutting edge technologies, and staffed by a CSMLS certified histotechnologist, the Histology Facility provides both routine and specialized histological support to the Lower Mainland research community.  Please contact the HLI Histology Facility for more information, project design assistance, and/or a cost estimate.

Services Available

  1. Processing and Embedding
    1. Paraffin embedding
    2. OCT embedding for cryosectioning
    3. Methylmethacrylate for electron microscopy
  2. Sectioning
    1. Paraffin
    2. Frozen
    3. Methylmethacrylate
    4. Decalcification (acid)
  3. Staining
    1. Histologic stains (see below)
    2. Immunohistochemistry (peroxidase and/or alkaline phosphatase)
    3. Immunofluorescence
    4. TUNEL staining
    5. In situ hybridization (ISH)
  4. Assay development and optimization

Histological Stains Available (sampling of stains available)

  1. General Morphology
    1. Hematoxylin and Eosin
    2. Toluidine Blue
    3. Giemsa
    4. TTC Staining
  2. Carbohydrates
    1. Periodic Acid-Schiff (PAS)
    2. PAS/diastase (glycogen)
    3. Alcian blue
    4. Alcian blue with PAS
  3. Connective Tissue
    1. Trichromes: Masson, Gomori
    2. Movat’s Pentachrome
    3. Gomori’s Aldehyde Fuchsin
    4. Verhoeff/Van Gieson
    5. Picrosirius Red
    6. Gomori’s silver impregnation
  4. Lipids
    1. Oil Red O
  5. Microorganisms
    1. Gram, Brown and Brenn
    2. Zeihl-Neelsen
    3. Grocott
  6. Nucleic Acids
    1. Feulgen
  7. Intracellular Granules
    1. Masson Fontana (melanin)
    2. Von Kossa (calcium)
    3. Amyloid
    4. Perl’s Prussian Blue (hemosiderin)
  8. Hematology
    1. Hansel’s (Eosinophils)
    2. Wright's
Contact Name: 
Histology
Contact Number: 
(604) 682-2344 x63546
Contact Email: 

hist...@hli.ubc.ca