Cellular Imaging and Biophysics Core

The Cellular Imaging & Biophysics Core at the Centre for Heart Lung Innovation provides services to individuals, institutions and industry in ultra high resolution imaging of cells and tissues in fixed and living specimens. Image analysis and consultations on microscopy related research projects are available upon request.

Confocal Microscopes

Our confocal microscopes can be used to 3-dimensionally image many biological systems and spatial distribution of fluorescently labeled proteins within a cell.

Our Zeiss LSM880 Inverted Confocal Microscope with superresolution is one of the most sophisticated research confocal microscopes. The microscope is capable of imaging 3-dimensional volumes with sub-micron resolution. The system features an Airyscan superresolution module that allows for instantaneous production of images to resolutions as small as 120 nm. This system is also equipped with a live cell temperature and humidity controlled incubation system, and a tunable Coherent Chameleon Ultra II laser capable of generating high-powered infrared laser pulses for multiphoton excitation.

Our Leica Upright Confocal Microscope is a filter-free spectral confocal and multiphoton microscope. The imaging operations which include selections of laser, detection channels and other functions are fully automated and computer controlled. This system features a nitrogen cooled stage for experiments on frozen tissues.

Micro Computed Tomography (micro CT) scanner

The Nikon micro CT scanner enables the micro-X-ray tomographic volume imaging of tissues and samples down to 1 micron, using the ultrahigh sensitivity PerkinElmer X-ray detector.

Wide Field Fluorescence Microscope

Wide Field Fluorescence Microscope and CCD camera which is capable of performing real-time, high speed imaging using an image splitter within the CCD camera that allows multi-wavelength fluorescence measurements to be taken in fixed and living cells.

Image Processing Stations

TThe Designated work stations are an excellent tool for 3D reconstruction and deconvolution of a series of 2D optical sections. The licensed Volocity software also enables the quantification and analysis of fluorescently labeled proteins or structures within an image.

Support

Our technicians have extensive training on all equipment and can provide support at every stage of your project from experimental design, protocol development, training and hands-on advice to data analysis.

Contact Name: 
Cellular Imaging & Biophysics Core
Contact Number: 
(604) 682-2344 x63419
Contact Email: 

c...@hli.ubc.ca